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Micropilot: automation of fluorescence microscopy-based imaging for systems biology.

ABSTRACT: Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.

SUBMITTER: Conrad C

PROVIDER: S-EPMC3086017 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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Micropilot: automation of fluorescence microscopy-based imaging for systems biology.

Conrad Christian C Wünsche Annelie A Tan Tze Heng TH Bulkescher Jutta J Sieckmann Frank F Verissimo Fatima F Edelstein Arthur A Walter Thomas T Liebel Urban U Pepperkok Rainer R Ellenberg Jan J

Nature methods 20110123 3

Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool f ...[more]

PMID: 21258339

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