High-throughput sequencing of the zebrafish antibody repertoire.
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ABSTRACT:
SUBMITTER: Weinstein JA
PROVIDER: S-EPMC3086368 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Publications
High-throughput sequencing of the zebrafish antibody repertoire.
Weinstein Joshua A JA Jiang Ning N White Richard A RA Fisher Daniel S DS Quake Stephen R SR
Science (New York, N.Y.) 20090501 5928
Despite tremendous progress in understanding the nature of the immune system, the full diversity of an organism's antibody repertoire is unknown. We used high-throughput sequencing of the variable domain of the antibody heavy chain from 14 zebrafish to analyze VDJ usage and antibody sequence. Zebrafish were found to use between 50 and 86% of all possible VDJ combinations and shared a similar frequency distribution, with some correlation of VDJ patterns between individuals. Zebrafish antibodies r ...[more]
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