Ontology highlight
ABSTRACT:
SUBMITTER: Hwang H
PROVIDER: S-EPMC3087643 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Hwang Heejin H Kim Sungsoo S Park Sohyun S Ngo Tri Duc TD Kim Kyeong Kyu KK Kim T Doohun TD
Acta crystallographica. Section F, Structural biology and crystallization communications 20110427 Pt 5
Industrial demand for active biocatalysts with desirable biochemical properties is constantly increasing and the discovery and characterization of novel esterases is potentially useful for industrial processes. Here, X-ray crystallographic studies of an (R)-specific SGNH arylesterase (Sm23) from Sinorhizobium meliloti 1021 are reported. The recombinant protein was expressed in Escherichia coli with a His tag and purified to homogeneity. Sm23 was crystallized using 0.2 M magnesium formate as a pr ...[more]