Ontology highlight
ABSTRACT:
SUBMITTER: Winther KS
PROVIDER: S-EPMC3088637 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20110418 18
Eukaryotic PIN (PilT N-terminal) domain proteins are ribonucleases involved in quality control, metabolism and maturation of mRNA and rRNA. The majority of prokaryotic PIN-domain proteins are encoded by the abundant vapBC toxin--antitoxin loci and inhibit translation by an unknown mechanism. Here we show that enteric VapCs are site-specific endonucleases that cleave tRNA(fMet) in the anticodon stem-loop between nucleotides +38 and +39 in vivo and in vitro. Consistently, VapC inhibited translatio ...[more]