Ontology highlight
ABSTRACT:
SUBMITTER: Jinek M
PROVIDER: S-EPMC3090138 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Jinek Martin M Coyle Scott M SM Doudna Jennifer A JA
Molecular cell 20110301 5
Messenger RNA decay plays a central role in the regulation and surveillance of eukaryotic gene expression. The conserved multidomain exoribonuclease Xrn1 targets cytoplasmic RNA substrates marked by a 5' monophosphate for processive 5'-to-3' degradation by an unknown mechanism. Here, we report the crystal structure of an Xrn1-substrate complex. The single-stranded substrate is held in place by stacking of the 5'-terminal trinucleotide between aromatic side chains while a highly basic pocket spec ...[more]