Ontology highlight
ABSTRACT:
SUBMITTER: Shortridge MD
PROVIDER: S-EPMC3090486 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Journal of proteome research 20110322 5
We report that proteins with the same function bind the same set of small molecules from a standardized chemical library. This observation led to a quantifiable and rapidly adaptable method for protein functional analysis using experimentally derived ligand binding profiles. Ligand binding is measured using a high-throughput NMR ligand affinity screen with a structurally diverse chemical library. The method was demonstrated using a set of 19 proteins with a range of functions. A statistically si ...[more]