Ontology highlight
ABSTRACT:
SUBMITTER: Zhang G
PROVIDER: S-EPMC3090507 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Zhang Guoan G Deinhardt Katrin K Chao Moses V MV Neubert Thomas A TA
Journal of proteome research 20110328 5
Conventional stable isotope labeling with amino acids in cell culture (SILAC) requires extensive metabolic labeling of proteins and therefore is difficult to apply to cells that do not divide or are unstable in SILAC culture. Using two different sets of heavy amino acids for labeling allows for straightforward SILAC quantitation using partially labeled cells because the two cell populations are always equally labeled. Here we report the application of this labeling strategy to primary cultured n ...[more]