Ontology highlight
ABSTRACT:
SUBMITTER: Chen C
PROVIDER: S-EPMC3090999 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Chen Chunlai C Stevens Benjamin B Kaur Jaskarin J Cabral Diana D Liu Hanqing H Wang Yuhong Y Zhang Haibo H Rosenblum Gabriel G Smilansky Zeev Z Goldman Yale E YE Cooperman Barry S BS
Molecular cell 20110501 3
We employ single-molecule fluorescence resonance energy transfer (smFRET) to study structural dynamics over the first two elongation cycles of protein synthesis, using ribosomes containing either Cy3-labeled ribosomal protein L11 and A- or P-site Cy5-labeled tRNA or Cy3- and Cy5-labeled tRNAs. Pretranslocation (PRE) complexes demonstrate fluctuations between classical and hybrid forms, with concerted motions of tRNAs away from L11 and from each other when classical complex converts to hybrid com ...[more]