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Transferrin-mediated targeting of bacteriophage HK97 nanoparticles into tumor cells.

ABSTRACT: Next-generation targeted nanodevices are currently under development for imaging and therapeutic applications. We engineered HK97 viral nanoparticles (VNPs) for tumor cell-specific targeting.A combination of genetic and chemical engineering methods were developed and applied to generate dual-labeled HK97 cysteine mutant particles displaying transferrin and fluorescent labels. The targeting properties of transferrin-conjugated VNPs were evaluated by in vitro experiments using different cancer cell lines.We found that HK97-transferrin formulations were indeed targeted to cancer cells in vitro via the transferrin receptor. These studies highlight the utility and facilitate the further development of HK97-based VNPs.

SUBMITTER: Huang RK

PROVIDER: S-EPMC3091364 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Transferrin-mediated targeting of bacteriophage HK97 nanoparticles into tumor cells.

Huang Rick K RK Steinmetz Nicole F NF Fu Chi-Yu CY Manchester Marianne M Johnson John E JE

Nanomedicine (London, England) 20110101 1

<h4>Aims</h4>Next-generation targeted nanodevices are currently under development for imaging and therapeutic applications. We engineered HK97 viral nanoparticles (VNPs) for tumor cell-specific targeting.<h4>Methods</h4>A combination of genetic and chemical engineering methods were developed and applied to generate dual-labeled HK97 cysteine mutant particles displaying transferrin and fluorescent labels. The targeting properties of transferrin-conjugated VNPs were evaluated by in vitro experimen ...[more]

PMID: 21182418

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