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Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis.

ABSTRACT: Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCR? chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease.

SUBMITTER: Biegler BW 

PROVIDER: S-EPMC3092854 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature

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Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis.

Biegler Brian W BW   Yan Shirley X SX   Ortega Sterling B SB   Tennakoon Deepani K DK   Racke Michael K MK   Karandikar Nitin J NJ  

Journal of neuroimmunology 20110311 1-2

Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to publi  ...[more]