Patterned resonance plasmonic microarrays for high-performance SPR imaging.

ABSTRACT: We report a novel optical platform based on SPR generation and confinement inside a defined three-dimensional microwell geometry that leads to background resonance-free SPR images. The array shows an exceptionally high signal-to-noise ratio (S/N > 80) for imaging analysis and subnanometric thickness resolution. An angular sensitivity of 1°/0.01 RIU has been achieved and the signal to background ratio (S/B) improves to 20, 1 order of magnitude higher than that of the best literature results. The design proves effective for probing-supported lipid membrane arrays in real time with a thickness resolution of 0.24 nm and allows for imaging analysis of microfluidic circuits where resonant spots are separated by only one pixel (?7 ?m). The high image quality and unique chip geometry open up new avenues for array screening and biomicrofluidics using SPRi detection.

SUBMITTER: Abbas A 

PROVIDER: S-EPMC3093414 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature