Protein signaling networks from single cell fluctuations and information theory profiling.
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ABSTRACT:
SUBMITTER: Shin YS
PROVIDER: S-EPMC3093549 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Publications
Protein signaling networks from single cell fluctuations and information theory profiling.
Shin Young Shik YS Remacle F F Fan Rong R Hwang Kiwook K Wei Wei W Ahmad Habib H Levine R D RD Heath James R JR
Biophysical journal 20110501 10
Protein signaling networks among cells play critical roles in a host of pathophysiological processes, from inflammation to tumorigenesis. We report on an approach that integrates microfluidic cell handling, in situ protein secretion profiling, and information theory to determine an extracellular protein-signaling network and the role of perturbations. We assayed 12 proteins secreted from human macrophages that were subjected to lipopolysaccharide challenge, which emulates the macrophage-based in ...[more]
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