Application of fiber-optic attenuated total reflection-FT-IR methods for in situ characterization of protein delivery systems in real time.

ABSTRACT: A fiber-optic coupled attenuated total reflection (ATR)-FT-IR spectroscopy technique was applied to the study of two different therapeutic delivery systems, acid degradable hydrogels and nanoparticles. Real time exponential release of a model protein, human serum albumin (HSA), was observed from two different polymeric hydrogels formulated with a pH sensitive cross-linker. Spectroscopic examination of nanoparticles formulated with an acid degradable polymer shell and encapsulated HSA exhibited vibrational signatures characteristic of both particle and payload when exposed to lowered pH conditions, demonstrating the ability of this methodology to simultaneously measure phenomena arising from a system with a mixture of components. In addition, thorough characterization of these pH sensitive

SUBMITTER: McFearin CL 

PROVIDER: S-EPMC3094485 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature