Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR.

ABSTRACT:

Background

Reference genes are commonly used as the endogenous normalisation measure for the relative quantification of target genes. The appropriate application of quantitative real-time PCR (RT-qPCR), however, requires the use of reference genes whose level of expression is not affected by the test, by general physiological conditions or by inter-individual variability. For this purpose, seven reference genes were investigated in tissues of the most important cereals (wheat, barley and oats). Titre of Barley yellow dwarf virus (BYDV) was determined in oats using relative quantification with different reference genes and absolute quantification, and the results were compared.

Results

The expression of seven potential reference genes was evaluated in tissues of 180 healthy,

SUBMITTER: Jarosova J 

PROVIDER: S-EPMC3095291 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature