Dataset Information

Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes.

ABSTRACT: There is a need for biomarkers to monitor the development and progression of type 1 DM. We analyzed mRNA expression levels for granzyme B, perforin, fas ligand, TNF-?, IFN-?, Foxp3, IL-10, TGF-?, IL-4, IL-6, IL-17, Activation-induced cytidine deaminase (AID) and Immunoglobulin G gamma chain (IgG) genes in peripheral blood of at-risk, new-onset and long-term type 1 DM , and healthy controls. The majority of the genes were suppressed in long-term type 1 DM compared to controls and new-onset patients. IFN-?, IL-4 and IL-10 mRNA levels were significantly higher in new-onset compared to at-risk and long-term groups. There was decreased mRNA expression for AID and IgG and up-regulation of IFN-? with age in controls. Data suggest an overall depressed immunity in long-term type 1 DM. Increased gene expression levels for IFN-?, IL-4 and IL-10 in new-onset patients from at-risk patients might be used as potential markers for progression of the disease.

SUBMITTER: Han D

PROVIDER: S-EPMC3096683 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes.

Han Dongmei D Leyva Carlos A CA Matheson Della D Mineo Davide D Messinger Shari S Blomberg Bonnie B BB Hernandez Ana A Meneghini Luigi F LF Allende Gloria G Skyler Jay S JS Alejandro Rodolfo R Pugliese Alberto A Kenyon Norma S NS

Clinical immunology (Orlando, Fla.) 20110224 3

There is a need for biomarkers to monitor the development and progression of type 1 DM. We analyzed mRNA expression levels for granzyme B, perforin, fas ligand, TNF-α, IFN-γ, Foxp3, IL-10, TGF-β, IL-4, IL-6, IL-17, Activation-induced cytidine deaminase (AID) and Immunoglobulin G gamma chain (IgG<gamma>) genes in peripheral blood of at-risk, new-onset and long-term type 1 DM , and healthy controls. The majority of the genes were suppressed in long-term type 1 DM compared to controls and new-onset ...[more]

PMID: 21414848

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Dataset Information

Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes.

Publications

Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes.

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