Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
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ABSTRACT:
SUBMITTER: Huang J
PROVIDER: S-EPMC3098247 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
Huang Jiaqi J Zheng Zongli Z Andersson Anders F AF Engstrand Lars L Ye Weimin W
PloS one 20110519 5
Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample. ...[more]
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