Ontology highlight
ABSTRACT:
SUBMITTER: Huang J
PROVIDER: S-EPMC3098247 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
PloS one 20110519 5
Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample. ...[more]