Unknown

Dataset Information

0

Helper-dependent adenovirus is superior to first-generation adenovirus for expressing transgenes in atherosclerosis-prone arteries.


ABSTRACT: Vascular gene transfer is a powerful tool for investigating and treating vascular diseases; however, its utility is limited by brevity of transgene expression and vector-associated inflammation. Helper-dependent adenovirus (HDAd), an advanced-generation adenovirus that lacks all viral genes, is superior to first-generation adenovirus (FGAd) in normal rabbit arteries. We compared HDAd to FGAd in arteries of cholesterol-fed rabbits, a model of early atherogenesis in which transgene expression might be decreased, and inflammation increased.Carotid arteries of chow- and cholesterol-fed rabbits were infused with FGAd, HDAd, or medium. HDAd expressed a transgene at least as well in arteries of cholesterol-fed rabbits as in arteries of chow-fed rabbits and expressed more durably than FGAd. In arteries of cholesterol-fed rabbits, HDAd stimulated less intimal growth, lipid deposition, and inflammation than FGAd. Neither vector affected phenylephrine-induced contraction or nitroprusside-mediated relaxation; however, both vectors decreased maximal acetylcholine-stimulated vasorelaxation. The relative absence of intimal growth in HDAd arteries could interfere with the utility of this model for testing atheroprotective genes; however, both coinfusion of FGAd and extension of cholesterol feeding yielded larger intimal lesions, on which atheroprotective genes could be tested.HDAd is superior to FGAd for expression of transgenes in atherosclerosis-prone arteries.

SUBMITTER: Jiang B 

PROVIDER: S-EPMC3098319 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Helper-dependent adenovirus is superior to first-generation adenovirus for expressing transgenes in atherosclerosis-prone arteries.

Jiang Bo B   Qian Kun K   Du Liang L   Luttrell Ian I   Chitaley Kanchan K   Dichek David A DA  

Arteriosclerosis, thrombosis, and vascular biology 20110331 6


<h4>Objective</h4>Vascular gene transfer is a powerful tool for investigating and treating vascular diseases; however, its utility is limited by brevity of transgene expression and vector-associated inflammation. Helper-dependent adenovirus (HDAd), an advanced-generation adenovirus that lacks all viral genes, is superior to first-generation adenovirus (FGAd) in normal rabbit arteries. We compared HDAd to FGAd in arteries of cholesterol-fed rabbits, a model of early atherogenesis in which transge  ...[more]

Similar Datasets

| S-EPMC1569481 | biostudies-literature
| S-EPMC8200741 | biostudies-literature
| S-EPMC2997586 | biostudies-literature
| S-EPMC3810942 | biostudies-literature
| S-EPMC3498883 | biostudies-literature
| S-EPMC5744068 | biostudies-literature
| S-EPMC2659436 | biostudies-literature
| S-EPMC4277965 | biostudies-literature
| S-EPMC5133319 | biostudies-literature
| S-EPMC6817846 | biostudies-literature