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Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine.

ABSTRACT: Kae1 is a universally conserved ATPase and part of the essential gene set in bacteria. In archaea and eukaryotes, Kae1 is embedded within the protein kinase-containing KEOPS complex. Mutation of KEOPS subunits in yeast leads to striking telomere and transcription defects, but the exact biochemical function of KEOPS is not known. As a first step to elucidating its function, we solved the atomic structure of archaea-derived KEOPS complexes involving Kae1, Bud32, Pcc1, and Cgi121 subunits. Our studies suggest that Kae1 is regulated at two levels by the primordial protein kinase Bud32, which is itself regulated by Cgi121. Moreover, Pcc1 appears to function as a dimerization module, perhaps suggesting that KEOPS may be a processive molecular machine. Lastly, as Bud32 lacks the conventional substrate-recognition infrastructure of eukaryotic protein kinases including an activation segment, Bud32 may provide a glimpse of the evolutionary history of the protein kinase family.

SUBMITTER: Mao DY

PROVIDER: S-EPMC3098719 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine.

Mao Daniel Y L DY Neculai Dante D Downey Michael M Orlicky Stephen S Haffani Yosr Z YZ Ceccarelli Derek F DF Ho Jenny S L JS Szilard Rachel K RK Zhang Wei W Ho Cynthia S CS Wan Leo L Fares Christophe C Rumpel Sigrun S Kurinov Igor I Arrowsmith Cheryl H CH Durocher Daniel D Sicheri Frank F

Molecular cell 20081001 2

Kae1 is a universally conserved ATPase and part of the essential gene set in bacteria. In archaea and eukaryotes, Kae1 is embedded within the protein kinase-containing KEOPS complex. Mutation of KEOPS subunits in yeast leads to striking telomere and transcription defects, but the exact biochemical function of KEOPS is not known. As a first step to elucidating its function, we solved the atomic structure of archaea-derived KEOPS complexes involving Kae1, Bud32, Pcc1, and Cgi121 subunits. Our stud ...[more]

PMID: 18951093

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Dataset Information

Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine.

Publications

Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine.

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