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In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.


ABSTRACT: We previously reported that fetal porcine skin-derived stem cells were capable of differentiation into oocyte-like cells (OLCs). Here we report that newborn mice skin-derived stem cells are also capable of differentiating into early OLCs. Using stem cells from mice that are transgenic for Oct4 germline distal enhancer-GFP, germ cells resulting from their differentiation are expected to be GFP(+). After differentiation, some GFP(+) OLCs reached 40-45 µM and expressed oocyte markers. Flow cytometric analysis revealed that ? 0.3% of the freshly isolated skin cells were GFP(+). The GFP-positive cells increased to ? 7% after differentiation, suggesting that the GFP(+) cells could be of in vivo origin, but are more likely induced upon being cultured in vitro. To study the in vivo germ cell potential of skin-derived cells, they were aggregated with newborn ovarian cells, and transplanted under the kidney capsule of ovariectomized mice. GFP(+) oocytes were identified within a subpopulation of follicles in the resulting growth. Our finding that early oocytes can be differentiated from mice skin-derived cells in defined medium may offer a new in vitro model to study germ cell formation and oogenesis.

SUBMITTER: Dyce PW 

PROVIDER: S-EPMC3101249 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.

Dyce Paul W PW   Liu Jinghe J   Tayade Chandrakant C   Kidder Gerald M GM   Betts Dean H DH   Li Julang J  

PloS one 20110524 5


We previously reported that fetal porcine skin-derived stem cells were capable of differentiation into oocyte-like cells (OLCs). Here we report that newborn mice skin-derived stem cells are also capable of differentiating into early OLCs. Using stem cells from mice that are transgenic for Oct4 germline distal enhancer-GFP, germ cells resulting from their differentiation are expected to be GFP(+). After differentiation, some GFP(+) OLCs reached 40-45 µM and expressed oocyte markers. Flow cytometr  ...[more]

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