Differential effects of thiopeptide and orthosomycin antibiotics on translational GTPases.
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ABSTRACT:
SUBMITTER: Mikolajka A
PROVIDER: S-EPMC3102230 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Publications
Differential effects of thiopeptide and orthosomycin antibiotics on translational GTPases.
Mikolajka Aleksandra A Liu Hanqing H Chen Yuanwei Y Starosta Agata L AL Márquez Viter V Ivanova Marina M Cooperman Barry S BS Wilson Daniel N DN
Chemistry & biology 20110501 5
The ribosome is a major target in the bacterial cell for antibiotics. Here, we dissect the effects that the thiopeptide antibiotics thiostrepton (ThS) and micrococcin (MiC) as well as the orthosomycin antibiotic evernimicin (Evn) have on translational GTPases. We demonstrate that, like ThS, MiC is a translocation inhibitor, and that the activation by MiC of the ribosome-dependent GTPase activity of EF-G is dependent on the presence of the ribosomal proteins L7/L12 as well as the G' subdomain of ...[more]
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