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Choosing and using a plant DNA barcode.


ABSTRACT: The main aim of DNA barcoding is to establish a shared community resource of DNA sequences that can be used for organismal identification and taxonomic clarification. This approach was successfully pioneered in animals using a portion of the cytochrome oxidase 1 (CO1) mitochondrial gene. In plants, establishing a standardized DNA barcoding system has been more challenging. In this paper, we review the process of selecting and refining a plant barcode; evaluate the factors which influence the discriminatory power of the approach; describe some early applications of plant barcoding and summarise major emerging projects; and outline tool development that will be necessary for plant DNA barcoding to advance.

SUBMITTER: Hollingsworth PM 

PROVIDER: S-EPMC3102656 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Choosing and using a plant DNA barcode.

Hollingsworth Peter M PM   Graham Sean W SW   Little Damon P DP  

PloS one 20110526 5


The main aim of DNA barcoding is to establish a shared community resource of DNA sequences that can be used for organismal identification and taxonomic clarification. This approach was successfully pioneered in animals using a portion of the cytochrome oxidase 1 (CO1) mitochondrial gene. In plants, establishing a standardized DNA barcoding system has been more challenging. In this paper, we review the process of selecting and refining a plant barcode; evaluate the factors which influence the dis  ...[more]

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