Microarrays, deep sequencing and the true measure of the transcriptome.
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ABSTRACT:
SUBMITTER: Malone JH
PROVIDER: S-EPMC3104486 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Publications
Microarrays, deep sequencing and the true measure of the transcriptome.
Malone John H JH Oliver Brian B
BMC biology 20110531
Microarrays first made the analysis of the transcriptome possible, and have produced much important information. Today, however, researchers are increasingly turning to direct high-throughput sequencing -- RNA-Seq -- which has considerable advantages for examining transcriptome fine structure -- for example in the detection of allele-specific expression and splice junctions. In this article, we discuss the relative merits of the two techniques, the inherent biases in each, and whether all of the ...[more]
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