Short-insert libraries as a method of problem solving in genome sequencing.
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ABSTRACT:
SUBMITTER: McMurray AA
PROVIDER: S-EPMC310723 | biostudies-literature | 1998 May
REPOSITORIES: biostudies-literature
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Short-insert libraries as a method of problem solving in genome sequencing.
McMurray A A AA Sulston J E JE Quail M A MA
Genome research 19980501 5
As the Human Genome Project moves into its sequencing phase, a serious problem has arisen. The same problem has been increasingly vexing in the closing phase of the Caenorhabditis elegans project. The difficulty lies in sequencing efficiently through certain regions in which the templates (DNA substrates for the sequencing process) form complex folded secondary structures that are inaccessible to the enzymes. The solution, however, is simply to break them up. Specifically, the offending fragment ...[more]
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