Unknown

Dataset Information

0

Peptide surfactants for cell-free production of functional G protein-coupled receptors.

ABSTRACT: Two major bottlenecks in elucidating the structure and function of membrane proteins are the difficulty of producing large quantities of functional receptors, and stabilizing them for a sufficient period of time. Selecting the right surfactant is thus crucial. Here we report using peptide surfactants in commercial Escherichia coli cell-free systems to rapidly produce milligram quantities of soluble G protein-coupled receptors (GPCRs). These include the human formyl peptide receptor, human trace amine-associated receptor, and two olfactory receptors. The GPCRs expressed in the presence of the peptide surfactants were soluble and had ?-helical secondary structures, suggesting that they were properly folded. Microscale thermophoresis measurements showed that one olfactory receptor expressed using peptide surfactants bound its known ligand heptanal (molecular weight 114.18). These short and simple peptide surfactants may be able to facilitate the rapid production of GPCRs, or even other membrane proteins, for structure and function studies.

SUBMITTER: Wang X 

PROVIDER: S-EPMC3107261 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Peptide surfactants for cell-free production of functional G protein-coupled receptors.

Wang Xiaoqiang X   Corin Karolina K   Baaske Philipp P   Wienken Christoph J CJ   Jerabek-Willemsen Moran M   Duhr Stefan S   Braun Dieter D   Zhang Shuguang S  

Proceedings of the National Academy of Sciences of the United States of America 20110511 22

Two major bottlenecks in elucidating the structure and function of membrane proteins are the difficulty of producing large quantities of functional receptors, and stabilizing them for a sufficient period of time. Selecting the right surfactant is thus crucial. Here we report using peptide surfactants in commercial Escherichia coli cell-free systems to rapidly produce milligram quantities of soluble G protein-coupled receptors (GPCRs). These include the human formyl peptide receptor, human trace  ...[more]

Similar Datasets

Unknown Cell-free synthesis of isotopically labelled peptide ligands for the functional characterization of G protein-coupled receptors.
| S-EPMC4794788 | biostudies-literature
Unknown Production of G protein-coupled receptors in an insect-based cell-free system.
| S-EPMC5599999 | biostudies-literature
Unknown Mapping functional group free energy patterns at protein occluded sites: nuclear receptors and G-protein coupled receptors.
| S-EPMC4372819 | biostudies-literature
Unknown Peptide-Liganded G Protein-Coupled Receptors as Neurotherapeutics.
| S-EPMC7155190 | biostudies-literature
Unknown Peptide ligand recognition by G protein-coupled receptors.
| S-EPMC4360564 | biostudies-literature
Unknown Understanding Peptide Binding in Class A G Protein-Coupled Receptors.
| S-EPMC6776014 | biostudies-literature
Unknown Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts.
| S-EPMC4766470 | biostudies-literature
Unknown Bioorthogonal fluorescent labeling of functional G-protein-coupled receptors.
| S-EPMC4215543 | biostudies-literature
Unknown Polymer-based cell-free expression of ligand-binding family B G-protein coupled receptors without detergents.
| S-EPMC3104232 | biostudies-literature
Unknown Discovery and validation of novel peptide agonists for G-protein-coupled receptors.
| S-EPMC3259865 | biostudies-literature