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Selective biomolecular nanoarrays for parallel single-molecule investigations.

ABSTRACT: The ability to direct the self-assembly of biomolecules on surfaces with true nanoscale control is key for the creation of functional substrates. Herein we report the fabrication of nanoscale biomolecular arrays via selective self-assembly on nanopatterned surfaces and minimized nonspecific adsorption. We demonstrate that the platform developed allows for the simultaneous screening of specific protein-DNA binding events at the single-molecule level. The strategy presented here is generally applicable and enables high-throughput monitoring of biological activity in real time and with single-molecule resolution.

SUBMITTER: Palma M

PROVIDER: S-EPMC3108261 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature

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Selective biomolecular nanoarrays for parallel single-molecule investigations.

Palma Matteo M Abramson Justin J JJ Gorodetsky Alon A AA Penzo Erika E Gonzalez Ruben L RL Sheetz Michael P MP Nuckolls Colin C Hone James J Wind Shalom J SJ

Journal of the American Chemical Society 20110429 20

The ability to direct the self-assembly of biomolecules on surfaces with true nanoscale control is key for the creation of functional substrates. Herein we report the fabrication of nanoscale biomolecular arrays via selective self-assembly on nanopatterned surfaces and minimized nonspecific adsorption. We demonstrate that the platform developed allows for the simultaneous screening of specific protein-DNA binding events at the single-molecule level. The strategy presented here is generally appli ...[more]

PMID: 21528859

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