Ontology highlight
ABSTRACT:
SUBMITTER: Rees JS
PROVIDER: S-EPMC3108830 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Rees Johanna S JS Lowe Nick N Armean Irina M IM Roote John J Johnson Glynnis G Drummond Emma E Spriggs Helen H Ryder Edward E Russell Steven S St Johnston Daniel D Lilley Kathryn S KS
Molecular & cellular proteomics : MCP 20110329 6
Affinity purification coupled to mass spectrometry provides a reliable method for identifying proteins and their binding partners. In this study we have used Drosophila melanogaster proteins triple tagged with Flag, Strep II, and Yellow fluorescent protein in vivo within affinity pull-down experiments and isolated these proteins in their native complexes from embryos. We describe a pipeline for determining interactomes by Parallel Affinity Capture (iPAC) and show its use by identifying partners ...[more]