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Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1).


ABSTRACT: The gene encoding T cell immunoglobulin and mucin domain-1 (Tim-1) is linked to atopy and asthma susceptibility in mice and humans. Tim-1 is a transmembrane protein expressed on activated lymphocytes and appears to have a role as a co-stimulatory receptor in T cells. The protein has not been shown to have enzymatic activity but contains a site within its cytoplasmic tail predicted to be a target for tyrosine kinases. Here, we show that Tim-1 can associate with the kinase Fyn, a member of the Src family of tyrosine kinases. This association does not require Fyn's kinase activity and is independent of the phosphorylation of a conserved tyrosine present within the cytoplasmic tail of Tim-1. Fyn is necessary for phosphorylation of this tyrosine in Tim-1 and the phosphorylation of Tim-1 varies with the levels of Fyn present in cells. These data suggest a role for Fyn in the signaling downstream of Tim-1.

SUBMITTER: Curtiss ML 

PROVIDER: S-EPMC3109159 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1).

Curtiss Miranda L ML   Hostager Bruce S BS   Stepniak Elizabeth E   Singh Melody M   Manhica Natalie N   Knisz Judit J   Traver Geri G   Rennert Paul D PD   Colgan John D JD   Rothman Paul B PB  

Molecular immunology 20110421 12-13


The gene encoding T cell immunoglobulin and mucin domain-1 (Tim-1) is linked to atopy and asthma susceptibility in mice and humans. Tim-1 is a transmembrane protein expressed on activated lymphocytes and appears to have a role as a co-stimulatory receptor in T cells. The protein has not been shown to have enzymatic activity but contains a site within its cytoplasmic tail predicted to be a target for tyrosine kinases. Here, we show that Tim-1 can associate with the kinase Fyn, a member of the Src  ...[more]

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