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Ratiometric temperature sensing with semiconducting polymer dots.

ABSTRACT: This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dye-Rhodamine B (RhB), whose emission intensity decreases with increasing temperature-within the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More importantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelength excitation and has a linear temperature sensing range that matches well with the physiologically relevant temperatures. We employed Pdot-RhB for measuring intracellular temperatures in a live-cell imaging mode. The exceptional brightness of Pdot-RhB allows this nanoscale temperature sensor to be used also as a fluorescent probe for cellular imaging.

SUBMITTER: Ye F

PROVIDER: S-EPMC3109500 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Ratiometric temperature sensing with semiconducting polymer dots.

Ye Fangmao F Wu Changfeng C Jin Yuhui Y Chan Yang-Hsiang YH Zhang Xuanjun X Chiu Daniel T DT

Journal of the American Chemical Society 20110511 21

This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dye-Rhodamine B (RhB), whose emission intensity decreases with increasing temperature-within the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More impo ...[more]

PMID: 21548583

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