Ontology highlight
ABSTRACT:
SUBMITTER: Konno H
PROVIDER: S-EPMC311029 | biostudies-literature | 2001 Feb
REPOSITORIES: biostudies-literature
Konno H H Fukunishi Y Y Shibata K K Itoh M M Carninci P P Sugahara Y Y Hayashizaki Y Y
Genome research 20010201 2
We developed computer-based methods for constructing a nonredundant mouse full-length cDNA library. Our cDNA library construction process comprises assessment of library quality, sequencing the 3' ends of inserts and clustering, and completing a re-array to generate a nonredundant library from a redundant one. After the cDNA libraries are generated, we sequence the 5' ends of the inserts to check the quality of the library; then we determine the sequencing priority of each library. Selected libr ...[more]