HC-Pro silencing suppressor significantly alters the gene expression profile in tobacco leaves and flowers.

ABSTRACT:

Background

RNA silencing is used in plants as a major defence mechanism against invasive nucleic acids, such as viruses. Accordingly, plant viruses have evolved to produce counter defensive RNA-silencing suppressors (RSSs). These factors interfere in various ways with the RNA silencing machinery in cells, and thereby disturb the microRNA (miRNA) mediated endogene regulation and induce developmental and morphological changes in plants. In this study we have explored these effects using previously characterized transgenic tobacco plants which constitutively express (under CaMV 35S promoter) the helper component-proteinase (HC-Pro) derived from a potyviral genome. The transcript levels of leaves and flowers of these plants were analysed using microarray techniques (Tobacco 4 × 44 k, A

SUBMITTER: Soitamo AJ 

PROVIDER: S-EPMC3111369 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature