Ontology highlight
ABSTRACT:
SUBMITTER: Sankaranarayanan K
PROVIDER: S-EPMC3111412 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Sankaranarayanan Krithivasan K Timofeeff Michael N MN Spathis Rita R Lowenstein Tim K TK Lum J Koji JK
PloS one 20110609 6
Fluid inclusions in evaporite minerals (halite, gypsum, etc.) potentially preserve genetic records of microbial diversity and changing environmental conditions of Earth's hydrosphere for nearly one billion years. Here we describe a robust protocol for surface sterilization and retrieval of DNA from fluid inclusions in halite that, unlike previously published methods, guarantees removal of potentially contaminating surface-bound DNA. The protocol involves microscopic visualization of cell structu ...[more]