Ontology highlight
ABSTRACT:
SUBMITTER: Iben JR
PROVIDER: S-EPMC3113579 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Iben James R JR Epstein Jonathan A JA Bayfield Mark A MA Bruinsma Monique W MW Hasson Samuel S Bacikova Dagmar D Ahmad Daniel D Rockwell Denise D Kittler Ellen L W EL Zapp Maria L ML Maraia Richard J RJ
Nucleic acids research 20110211 11
We used a genetic screen based on tRNA-mediated suppression (TMS) in a Schizosaccharomyces pombe La protein (Sla1p) mutant. Suppressor pre-tRNA(Ser)UCA-C47:6U with a debilitating substitution in its variable arm fails to produce tRNA in a sla1-rrm mutant deficient for RNA chaperone-like activity. The parent strain and spontaneous mutant were analyzed using Solexa sequencing. One synonymous single-nucleotide polymorphism (SNP), unrelated to the phenotype, was identified. Further sequence analyses ...[more]