Ontology highlight
ABSTRACT:
SUBMITTER: Liang XH
PROVIDER: S-EPMC3113584 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Liang Xue-Hai XH Crooke Stanley T ST
Nucleic acids research 20110214 11
Little is known about whether components of the RNA-induced silencing complex (RISC) mediate the biogenesis of RNAs other than miRNA. Here, we show that depletion of key proteins of the RISC pathway by antisense oligonucleotides significantly impairs pre-rRNA processing in human cells. In cells depleted of Drosha or Dicer, different precursors to 5.8S rRNA strongly accumulated, without affecting normal endonucleolytic cleavages. Moderate yet distinct processing defects were also observed in Ago2 ...[more]