Abacus: a computational tool for extracting and pre-processing spectral count data for label-free quantitative proteomic analysis.
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ABSTRACT:
SUBMITTER: Fermin D
PROVIDER: S-EPMC3113614 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Publications
Abacus: a computational tool for extracting and pre-processing spectral count data for label-free quantitative proteomic analysis.
Fermin Damian D Basrur Venkatesha V Yocum Anastasia K AK Nesvizhskii Alexey I AI
Proteomics 20110217 7
We describe Abacus, a computational tool for extracting spectral counts from MS/MS data sets. The program aggregates data from multiple experiments, adjusts spectral counts to accurately account for peptides shared across multiple proteins, and performs common normalization steps. It can also output the spectral count data at the gene level, thus simplifying the integration and comparison between gene and protein expression data. Abacus is compatible with the widely used Trans-Proteomic Pipeline ...[more]
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