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Arginine methylation of G3BP1 in response to Wnt3a regulates ?-catenin mRNA.

ABSTRACT: Wnt/?-catenin signaling is essential for normal mammalian development. Wnt3a activates the Wnt/?-catenin pathway through stabilization of ?-catenin; a process in which the phosphoprotein Dishevelled figures prominently. Protein arginine methylation in signaling complexes containing Dishevelled was investigated. Mass spectrometry of a prominent arginine-methylated, Dishevelled-associated protein identified the Ras GTPase activating protein-binding protein 1 G3BP1. Stimulation of totipotent mouse embryonic F9 cells with Wnt3a provoked increased methylation of G3BP1. We show that G3BP1 is a novel Ctnnb1 mRNA binding protein. Methylation of G3BP1 constitutes a molecular switch that regulates Ctnnb1 mRNA in response to Wnt3a. Thus, the protein arginine methylation that targets G3BP1 acts as a novel regulator of Ctnnb1 mRNA.

SUBMITTER: Bikkavilli RK

PROVIDER: S-EPMC3113675 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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Arginine methylation of G3BP1 in response to Wnt3a regulates β-catenin mRNA.

Bikkavilli Rama Kamesh RK Malbon Craig C CC

Journal of cell science 20110607 Pt 13

Wnt/β-catenin signaling is essential for normal mammalian development. Wnt3a activates the Wnt/β-catenin pathway through stabilization of β-catenin; a process in which the phosphoprotein Dishevelled figures prominently. Protein arginine methylation in signaling complexes containing Dishevelled was investigated. Mass spectrometry of a prominent arginine-methylated, Dishevelled-associated protein identified the Ras GTPase activating protein-binding protein 1 G3BP1. Stimulation of totipotent mouse ...[more]

PMID: 21652632

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