A protein network-guided screen for cell cycle regulators in Drosophila.

ABSTRACT:

Background

Large-scale RNAi-based screens are playing a critical role in defining sets of genes that regulate specific cellular processes. Numerous screens have been completed and in some cases more than one screen has examined the same cellular process, enabling a direct comparison of the genes identified in separate screens. Surprisingly, the overlap observed between the results of similar screens is low, suggesting that RNAi screens have relatively high levels of false positives, false negatives, or both.

Results

We re-examined genes that were identified in two previous RNAi-based cell cycle screens to identify potential false positives and false negatives. We were able to confirm many of the originally observed phenotypes and to reveal many likely false positives. To ide

SUBMITTER: Guest ST 

PROVIDER: S-EPMC3113730 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature