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Investigation of MADS domain transcription factor dynamics in the floral meristem.

ABSTRACT: To study the importance of intercellular transport for MADS domain transcription factor functioning during floral development, we analyzed the dynamic behavior of fluorescently-tagged MADS domain proteins in transgenic plants by Confocal Laser Scanning Microscopy. These analyses, described in a recent paper in The Plant Journal, provided proof for previous suggestions that the Arabidopsis thaliana C-type protein AGAMOUS has a non-cell-autonomous role in floral meristem integrity. Furthermore, it indicated a possible non-cell-autonomous role for the B-type proteins APETALA3 and PISTILLATA, and the E-type protein SEPALLATA3, through lateral intercellular movement in the floral meristem. In this addendum we compare some of the available fluorescent protein-based technologies for the investigation of transcription factor movements and dynamics.

SUBMITTER: Urbanus SL

PROVIDER: S-EPMC3115362 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Investigation of MADS domain transcription factor dynamics in the floral meristem.

Urbanus Susan L SL Dinh Q D Peter QD Angenent Gerco C GC Immink Richard G H RG

Plant signaling & behavior 20101001 10

To study the importance of intercellular transport for MADS domain transcription factor functioning during floral development, we analyzed the dynamic behavior of fluorescently-tagged MADS domain proteins in transgenic plants by Confocal Laser Scanning Microscopy. These analyses, described in a recent paper in The Plant Journal, provided proof for previous suggestions that the Arabidopsis thaliana C-type protein AGAMOUS has a non-cell-autonomous role in floral meristem integrity. Furthermore, it ...[more]

PMID: 20861681

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