Ontology highlight
ABSTRACT:
SUBMITTER: Lang J
PROVIDER: S-EPMC3115423 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Lang Julie J Weiss Nicholas N Freed Brian M BM Torres Raul M RM Pelanda Roberta R
Clinical immunology (Orlando, Fla.) 20110414 1
Hematopoietic humanized mice generated via transplantation of human hematopoietic stem cells (hHSCs) into immunodeficient mice are a valuable tool for studying development and function of the human immune system. This study was performed to generate a protocol that improves development and quality of humanized mice in the BALB/c-Rag2(null)Il2rγ(null) strain, testing route of injection, in vitro culture and freezing of hHSCs, types of cytokines in the culture, and co-injection of lineage-depleted ...[more]