Unknown

Dataset Information

0

Genetic diversity and phylogeny of antagonistic bacteria against Phytophthora nicotianae isolated from tobacco rhizosphere.


ABSTRACT: The genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by BOXAIR-PCR, 16S-RFLP, 16S rRNA sequence homology and phylogenetic analysis methods. These studies revealed that 4.01% of the 6652 tested had some inhibitory activity against Phytophthora nicotianae. BOXAIR-PCR analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. A total of 25 16S-RFLP patterns were identified representing over 33 species from 17 different genera. Our results also found a significant amount of bacterial diversity among the antagonistic bacteria compared to other published reports. For the first time; Delftia tsuruhatensis, Stenotrophomonas maltophilia, Advenella incenata, Bacillus altitudinis, Kocuria palustris, Bacillus licheniformis, Agrobacterium tumefaciens and Myroides odoratimimus are reported to display antagonistic activity towards Phytophthora nicotianae. Furthermore, the majority (75%) of the isolates assayed for antagonistic activity were Gram-positives compared to only 25% that were Gram-negative bacteria.

SUBMITTER: Jin F 

PROVIDER: S-EPMC3116175 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

altmetric image

Publications

Genetic diversity and phylogeny of antagonistic bacteria against Phytophthora nicotianae isolated from tobacco rhizosphere.

Jin Fengli F   Ding Yanqin Y   Ding Wei W   Reddy M S MS   Fernando W G Dilantha WG   Du Binghai B  

International journal of molecular sciences 20110512 5


The genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by BOXAIR-PCR, 16S-RFLP, 16S rRNA sequence homology and phylogenetic analysis methods. These studies revealed that 4.01% of the 6652 tested had some inhibitory activity against Phytophthora nicotianae. BOXAIR-PCR analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. A total of 25 16S-RFLP patterns were iden  ...[more]

Similar Datasets

| S-EPMC5428407 | biostudies-literature
| S-EPMC3769708 | biostudies-literature
| S-EPMC9644278 | biostudies-literature
| S-EPMC5562454 | biostudies-literature
| S-EPMC4171770 | biostudies-literature
| S-EPMC8699867 | biostudies-literature
2022-03-09 | GSE168516 | GEO
| S-EPMC5752661 | biostudies-literature
| S-EPMC6640254 | biostudies-literature
| S-EPMC9277107 | biostudies-literature