Cytochrome P450: taming a wild type enzyme.
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ABSTRACT:
SUBMITTER: Jung ST
PROVIDER: S-EPMC3118264 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Publications
Cytochrome P450: taming a wild type enzyme.
Jung Sang Taek ST Lauchli Ryan R Arnold Frances H FH
Current opinion in biotechnology 20110314 6
Protein engineering of cytochrome P450 monooxygenases (P450s) has been very successful in generating valuable non-natural activities and properties, allowing these powerful catalysts to be used for the synthesis of drug metabolites and in biosynthetic pathways for the production of precursors of artemisinin and paclitaxel. Collected experience indicates that the P450s are highly 'evolvable' - they are particularly robust to mutation in their active sites and readily accept new substrates and exh ...[more]
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