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Molecular identification of Taenia tapeworms by Cox1 gene in Koh Kong, Cambodia.

ABSTRACT: We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples. Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, Taenia saginata, and Taenia asiatica based on 706, 629, and 474 bp bands, respectively. All of the Taenia specimens from Kho Kong, Cambodia, were identified as either T. saginata (n=19) or T. solium (n=2) by cox1 sequencing and multiplex PCR.

SUBMITTER: Jeon HK

PROVIDER: S-EPMC3121081 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Molecular identification of Taenia tapeworms by Cox1 gene in Koh Kong, Cambodia.

Jeon Hyeong-Kyu HK Yong Tai-Soon TS Sohn Woon-Mok WM Chai Jong-Yil JY Hong Sung-Jong SJ Han Eun-Taek ET Jeong Hoo-Gn HG Chhakda Tep T Sinuon Muth M Socheat Duong D Eom Keeseon S KS

The Korean journal of parasitology 20110614 2

We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples. Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, ...[more]

PMID: 21738280

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