Ontology highlight
ABSTRACT:
SUBMITTER: Dolan KT
PROVIDER: S-EPMC3121362 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Dolan Kyle T KT Duguid Erica M EM He Chuan C
The Journal of biological chemistry 20110505 25
SlyA is a master virulence regulator that controls the transcription of numerous genes in Salmonella enterica. We present here crystal structures of SlyA by itself and bound to a high-affinity DNA operator sequence in the slyA gene. SlyA interacts with DNA through direct recognition of a guanine base by Arg-65, as well as interactions between conserved Arg-86 and the minor groove and a large network of non-base-specific contacts with the sugar phosphate backbone. Our structures, together with an ...[more]