Ontology highlight
ABSTRACT:
SUBMITTER: Varley ZK
PROVIDER: S-EPMC3121485 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Varley Zeynep Kasap ZK Pizzarelli Rocco R Antonelli Roberta R Stancheva Stefka H SH Kneussel Matthias M Cherubini Enrico E Zacchi Paola P
The Journal of biological chemistry 20110420 23
Gephyrin is a scaffold protein essential for stabilizing glycine and GABA(A) receptors at inhibitory synapses. Here, recombinant intrabodies against gephyrin (scFv-gephyrin) were used to assess whether this protein exerts a transynaptic action on GABA and glutamate release. Pair recordings from interconnected hippocampal cells in culture revealed a reduced probability of GABA release in scFv-gephyrin-transfected neurons compared with controls. This effect was associated with a significant decrea ...[more]