Light control of plasma membrane recruitment using the Phy-PIF system.
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ABSTRACT:
SUBMITTER: Toettcher JE
PROVIDER: S-EPMC3121701 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
Light control of plasma membrane recruitment using the Phy-PIF system.
Toettcher Jared E JE Gong Delquin D Lim Wendell A WA Weiner Orion D OD
Methods in enzymology 20110101
The ability to control the activity of intracellular signaling processes in live cells would be an extraordinarily powerful tool. Ideally, such an intracellular input would be (i) genetically encoded, (ii) able to be turned on and off in defined temporal or spatial patterns, (iii) fast to switch between on and off states, and (iv) orthogonal to other cellular processes. The light-gated interaction between fragments of two plant proteins--termed Phy and PIF--satisfies each of these constraints. I ...[more]
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