Ontology highlight
ABSTRACT:
SUBMITTER: Ehrensberger AH
PROVIDER: S-EPMC3121832 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Ehrensberger Andreas H AH Kornberg Roger D RD
Proceedings of the National Academy of Sciences of the United States of America 20110606 25
Repressed PHO5 gene chromatin, isolated from yeast in the native state, was remodeled by yeast extract in a gene activator-dependent, ATP-dependent manner. The product of the reaction bore the hallmark of the process in vivo, the selective removal of promoter nucleosomes, without effect on open reading frame nucleosomes. Fractionation of the extract identified a single protein, chromodomain helicase DNA binding protein 1 (Chd1), capable of the remodeling activity. Deletion of the CHD1 gene in an ...[more]