Stress induced ? subunit of heterotrimeric G-proteins from Pisum sativum interacts with mitogen activated protein kinase.

ABSTRACT: We here report in Pisum sativum system a novel protein-protein interaction of ?-subunit of heterotrimeric G-proteins (PsG?) with a Mitogen activated protein kinase (PsMPK3) during cDNA library screening by yeast-two-hybrid assay. The transcript of these two genes also showed co-regulation under abscisic acid (ABA) and methyl jasmonate (MeJA) treatments. The protein-protein interaction was further validated by performing one-to-one interaction and ?-galactosidase assay in yeast system. ?-subunit of G-proteins from a heterologous system Oryzae sativa also showed interaction with PsMPK3. The interaction between PsG? and PsMPK3 was further confirmed by in vitro protein-protein interaction. This suggested that MPK3 function as effector molecule for G?, which may helps in the regulation of stomatal functioning. These findings also provide an evidence for a possible cross-talk between MPK3 and G-protein-mediated signaling pathways in plants.

SUBMITTER: Bhardwaj D 

PROVIDER: S-EPMC3121990 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature