Unknown

Dataset Information

0

The pentatricopeptide repeat protein OTP87 is essential for RNA editing of nad7 and atp1 transcripts in Arabidopsis mitochondria.


ABSTRACT: In plant organelles, RNA editing is a post-transcriptional mechanism that converts specific cytidines to uridines in RNA of both mitochondria and plastids, altering the information encoded by the gene. The cytidine to be edited is determined by a cis-element surrounding the editing site that is specifically recognized and bound by a trans-acting factor. All the trans-acting editing factors identified so far in plant organelles are members of a large protein family, the pentatricopeptide repeat (PPR) proteins. We have identified the Organelle Transcript Processing 87 (OTP87) gene, which is required for RNA editing of the nad7-C24 and atp1-C1178 sites in Arabidopsis mitochondria. OTP87 encodes an E-subclass PPR protein with an unusually short E-domain. The recombinant protein expressed in Escherichia coli specifically binds to RNAs comprising 30 nucleotides upstream and 10 nucleotides downstream of the nad7-C24 and atp1-C1178 editing sites. The loss-of-function of OTP87 results in small plants with growth and developmental delays. In the otp87 mutant, the amount of assembled respiratory complex V (ATP synthase) is highly reduced compared with the wild type suggesting that the amino acid alteration in ATP1 caused by loss of editing at the atp1-C1178 site affects complex V assembly in mitochondria.

SUBMITTER: Hammani K 

PROVIDER: S-EPMC3122196 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

The pentatricopeptide repeat protein OTP87 is essential for RNA editing of nad7 and atp1 transcripts in Arabidopsis mitochondria.

Hammani Kamel K   des Francs-Small Catherine Colas CC   Takenaka Mizuki M   Tanz Sandra K SK   Okuda Kenji K   Shikanai Toshiharu T   Brennicke Axel A   Small Ian I  

The Journal of biological chemistry 20110419 24


In plant organelles, RNA editing is a post-transcriptional mechanism that converts specific cytidines to uridines in RNA of both mitochondria and plastids, altering the information encoded by the gene. The cytidine to be edited is determined by a cis-element surrounding the editing site that is specifically recognized and bound by a trans-acting factor. All the trans-acting editing factors identified so far in plant organelles are members of a large protein family, the pentatricopeptide repeat (  ...[more]

Similar Datasets

| S-EPMC4962808 | biostudies-other
| S-EPMC3711453 | biostudies-literature
| S-EPMC7303307 | biostudies-literature
| S-EPMC8458969 | biostudies-literature
| S-EPMC7926422 | biostudies-literature
| S-EPMC9304396 | biostudies-literature
| S-EPMC3675099 | biostudies-literature
| S-EPMC5972581 | biostudies-literature
| S-EPMC1876591 | biostudies-literature
| S-EPMC6899631 | biostudies-literature