Ontology highlight
ABSTRACT:
SUBMITTER: Barbieri MR
PROVIDER: S-EPMC3122308 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Barbieri M Rosario MR Larosa Véronique V Nouet Cécile C Subrahmanian Nitya N Remacle Claire C Hamel Patrice P PP
Genetics 20110405 2
Mitochondrial complex I is the largest multimeric enzyme of the respiratory chain. The lack of a model system with facile genetics has limited the molecular dissection of complex I assembly. Using Chlamydomonas reinhardtii as an experimental system to screen for complex I defects, we isolated, via forward genetics, amc1-7 nuclear mutants (for assembly of mitochondrial complex I) displaying reduced or no complex I activity. Blue native (BN)-PAGE and immunoblot analyses revealed that amc3 and amc4 ...[more]