Effects of different centrifugation conditions on clinical chemistry and Immunology test results.

ABSTRACT:

Background

The effect of centrifugation time of heparinized blood samples on clinical chemistry and immunology results has rarely been studied. WHO guideline proposed a 15 min centrifugation time without citing any scientific publications. The centrifugation time has a considerable impact on the turn-around-time.

Methods

We investigated 74 parameters in samples from 44 patients on a Roche Cobas 6000 system, to see whether there was a statistical significant difference in the test results among specimens centrifuged at 2180 g for 15 min, at 2180 g for 10 min or at 1870 g for 7 min, respectively. Two tubes with different plasma separators (both Greiner Bio-One) were used for each centrifugation condition. Statistical comparisons were made by Deming fit.

Results

Tubes wi

SUBMITTER: Minder EI 

PROVIDER: S-EPMC3123263 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature