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Metabolic engineering of Clostridium cellulolyticum for production of isobutanol from cellulose.


ABSTRACT: Producing biofuels directly from cellulose, known as consolidated bioprocessing, is believed to reduce costs substantially compared to a process in which cellulose degradation and fermentation to fuel are accomplished in separate steps. Here we present a metabolic engineering example for the development of a Clostridium cellulolyticum strain for isobutanol synthesis directly from cellulose. This strategy exploits the host's natural cellulolytic activity and the amino acid biosynthesis pathway and diverts its 2-keto acid intermediates toward alcohol synthesis. Specifically, we have demonstrated the first production of isobutanol to approximately 660 mg/liter from crystalline cellulose by using this microorganism.

SUBMITTER: Higashide W 

PROVIDER: S-EPMC3126361 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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Metabolic engineering of Clostridium cellulolyticum for production of isobutanol from cellulose.

Higashide Wendy W   Li Yongchao Y   Yang Yunfeng Y   Liao James C JC  

Applied and environmental microbiology 20110304 8


Producing biofuels directly from cellulose, known as consolidated bioprocessing, is believed to reduce costs substantially compared to a process in which cellulose degradation and fermentation to fuel are accomplished in separate steps. Here we present a metabolic engineering example for the development of a Clostridium cellulolyticum strain for isobutanol synthesis directly from cellulose. This strategy exploits the host's natural cellulolytic activity and the amino acid biosynthesis pathway an  ...[more]

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